Reconstitution of apo-DT-diaphorase with flavin-adenine dinucleotide.
نویسندگان
چکیده
منابع مشابه
Combination with Flavin Adenine Dinucleotide*
The apoprotein of D-amino acid oxidase has been prepared by a new method involving dialysis of the holoenzyme against 1 M potassium bromide. The apoprotein prepared in this way has been found to have the same properties as that prepared by the classical acid-ammonium sulfate method. The effect of mixing apoprotein with flavin adenine dinucleotide has been followed by changes in absorbance at 49...
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The method described in the present work allows the purity of the solution under test for tocopherols to be assessed and also enables the separation of the commonly occurring a-, yand 8-tocopherols to be achieved. Applications of the method to the examination of several oils indicate the usefulness of the method. For example, the presence of 8tocopherol in soya-bean oil is readily ascertained, ...
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An enzyme has been partially purified from brewer's yeast which catalyzes the reversible reaction between riboflavin phosphate and adenosine triphosphate (ATP) on the one hand and flavin-adenine dinucleotide (FAD) and inorganic pyrophosphate on the other in the presence of magnesium ions. ATP could not be replaced by adenosine diphosphate or adenosine-5-phosphate, and in the reverse reaction, i...
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The reconstitution reaction of glucose oxidase from FAD and apoenzyme was studied : (1) Unimolecular reaction takes part in the reaction of glucose apooxidase with FAD as a rate limiting step. (2) At the very initial stage of the reaction, intensity of the fluorescence derived from FAD was observed to increase rapidly and to be quenched slowly after reaching the maximum state. (3) When glucose ...
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Data obtained by means of proton magnetic resonance spectroscopy indicate that specific association of FMN occurs with AMP in aqueous solution, because much weaker interaction is observed with FMN of either GMP or CMP. A comparison of FAD with a 1:1 mixture of FMN and AMP suggests that the flavin is involved in an intramolecular hydrogen bonding with the adenine moiety of FAD. The temperature d...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1986
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.34.4840